s12283
NM_001272043.1
NM_001272044.1
NM_001272060.1
NM_003161.3
XM_011525101.2
XM_011525102.2
XM_011525103.2
XM_017024929.1
XM_017024930.1
XM_017024931.1
XM_017024932.1
XM_017024933.1
8%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177357_m1]
HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.