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SiRNA Validation Data

siRNA ID:

s20564

Gene ID:

10492

Transcript(s):

NM_001159673.1 NM_001159674.1 NM_001159675.1 NM_001159676.1 NM_001159677.1 NM_001253771.1 NM_006372.4 XM_005248635.4 XM_005248636.2 XM_005248637.2 XM_017010176.1 XM_017010177.1 XM_017010178.1 XM_017010179.1 XM_017010180.1

Validation Results:

14%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00749309_s1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.