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SiRNA Validation Data

siRNA ID:

s5548

Gene ID:

2588

Transcript(s):

NM_000512.4 NM_001323543.1 NM_001323544.1 XM_005256301.3 XM_011522982.2 XM_017023111.1 XM_017023112.1 XM_017023113.1

Validation Results:

9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00166833_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.