SiRNA Validation Data



Gene ID:



NM_001033581.1 NM_001033582.1 NM_001242874.1 NM_002744.4 XM_011541773.1 XM_011541774.1 XM_011541775.2 XM_011541776.1 XM_011541778.1 XM_017001789.1 XM_017001790.1 XM_017001791.1 XM_017001792.1 XM_017001793.1 XM_017001794.1 XM_017001795.1 XM_017001796.1 XM_017001798.1 XM_017001799.1 XM_017001802.1 XM_017001803.1 XM_017001804.1 XM_017001805.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177051_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.