SiRNA Validation Data

siRNA ID:

s1506

Gene ID:

355

Transcript(s):

NM_000043.5 NM_001320619.1 NM_152871.3 NM_152872.3 NR_028033.3 NR_028034.3 NR_028035.3 NR_028036.3 NR_135313.1 NR_135314.1 NR_135315.1 XM_006717819.3 XM_011539764.2 XM_011539765.2 XM_011539766.2 XM_011539767.2

Validation Results:

16%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00531110_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.