SiRNA Validation Data



Gene ID:



NM_001242466.1 NM_181504.3 NM_181523.2 NM_181524.1 XM_005248542.3 XM_011543493.2 XM_017009585.1 XM_017009586.1

Validation Results:

14%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00381459_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.