SiRNA Validation Data

siRNA ID:

s4244

Gene ID:

1798

Transcript(s):

NM_001382.3 XM_005271422.2 XM_011542648.1 XM_017017293.1 XM_017017294.1

Validation Results:

12%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00609752_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.