SiRNA Validation Data



Gene ID:



NM_003872.2 NM_018534.3 NM_201264.1 NM_201266.1 NM_201267.1 NM_201279.1 XM_005246933.3 XM_005246934.3 XM_017005185.1 XM_017005186.1 XM_017005187.1 XM_017005188.1

Validation Results:

11%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00187290_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.