SiRNA Validation Data

siRNA ID:

6031

Gene ID:

Transcript(s):

NM_001253727.1 NM_001253729.1 NM_001253732.1 NM_001253733.1 NM_021634.3 NR_045579.1 NR_045580.1 NR_045581.1 NR_045582.1 NR_045583.1 NR_045584.1 XM_011532174.1 XM_011532175.1 XM_011532176.1 XM_011532179.2 XM_017008517.1 XM_017008518.1 XM_017008519.1 XM_017008520.1 XM_017008521.1 XM_017008522.1 XM_017008523.1 XM_017008524.1

Validation Results:

9%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00222171_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.