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SiRNA Validation Data

siRNA ID:

1197

Gene ID:

83549

Transcript(s):

NM_001135954.2 NM_001261450.2 NM_001261451.2 NM_001318519.1 NM_031432.3 NR_134666.1 NR_134667.1 XM_005272224.2

Validation Results:

29%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.