SiRNA Validation Data



Gene ID:



NM_001135821.1 NM_001135822.1 NM_001242824.1 NM_001242825.1 NM_002004.3 XM_005244962.1 XM_005244963.1

Validation Results:

29%(+/-8% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs01578769_g1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.