SiRNA Validation Data

siRNA ID:

s32330

Gene ID:

56894

Transcript(s):

NM_001037553.1 NM_020132.4 XM_005261160.4 XM_006724029.3 XM_006724030.3 XM_006724031.3 XM_011529660.2 XM_011529661.2 XM_011529662.2 XM_011529663.2 XM_011529664.2 XM_011529665.2 XM_017028408.1 XM_017028409.1 XM_017028410.1 XM_017028411.1 XM_017028412.1 XM_017028413.1

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00220017_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.