SiRNA Validation Data

siRNA ID:

103355

Gene ID:

51592

Transcript(s):

NM_015906.3 NM_033020.2 XM_005270936.3 XM_005270937.3 XM_011541568.2 XM_017001452.1 XM_017001453.1 XM_017001454.1

Validation Results:

13%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00210808_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.