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SiRNA Validation Data

siRNA ID:

114058

Gene ID:

351

Transcript(s):

NM_000484.3 NM_001136016.3 NM_001136129.2 NM_001136130.2 NM_001136131.2 NM_001204301.1 NM_001204302.1 NM_001204303.1 NM_201413.2 NM_201414.2

Validation Results:

6%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00169098_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.