SiRNA Validation Data

siRNA ID:

118375

Gene ID:

Transcript(s):

NM_001301226.1 NM_001301227.1 NM_003289.3 NM_213674.1 XM_017015087.1 XM_017015088.1 XM_017015089.1 XM_017015090.1 XM_017015091.1 XM_017015092.1 XM_017015093.1

Validation Results:

10%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00268540_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.