SiRNA Validation Data

siRNA ID:

118564

Gene ID:

4281

Transcript(s):

NM_000381.3 NM_001098624.2 NM_001193277.1 NM_001193278.1 NM_001193279.1 NM_001193280.1 NM_033289.1 NM_033290.3 XM_005274536.1 XM_005274537.1 XM_006724492.3 XM_011545525.2 XM_011545526.2 XM_011545527.2 XM_017029534.1 XM_017029535.1 XM_017029536.1 XM_017029537.1 XM_017029538.1 XM_017029539.1 XM_017029540.1 XM_017029541.1 XM_017029542.1

Validation Results:

9%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00166057_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.