SiRNA Validation Data

siRNA ID:

122221

Gene ID:

2182

Transcript(s):

NM_001318509.1 NM_001318510.1 NM_004458.2 NM_022977.2 XM_005262109.2 XM_006724635.2 XM_011530888.2 XM_011530889.2

Validation Results:

17%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00244871_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.