Close X

SiRNA Validation Data

siRNA ID:

122592

Gene ID:

10256

Transcript(s):

NM_001297647.1 NM_001297648.1 NM_006314.2 XM_011540484.2 XM_011540485.2

Validation Results:

18%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00178995_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.