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SiRNA Validation Data

siRNA ID:

213182

Gene ID:

7109

Transcript(s):

NM_003274.4 XM_005261168.4 XM_011529714.2 XM_011529715.2 XM_011529716.2 XM_011529717.2 XM_011529718.2 XM_011529720.2 XM_011529725.2 XM_011529726.2 XM_011529728.2 XM_011529729.2 XM_011529730.2 XM_017028454.1

Validation Results:

16%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00162815_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.