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SiRNA Validation Data

siRNA ID:

277

Gene ID:

5211

Transcript(s):

NM_001002021.2 NM_002626.5 XM_005261135.3 XM_005261137.4 XM_006724011.1 XM_006724012.1 XM_017028368.1 XM_017028369.1 XM_017028370.1 XM_017028371.1 XM_017028372.1

Validation Results:

10%(+/-0% mRNA remaining)(DU145 cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.