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SiRNA Validation Data

siRNA ID:

37190

Gene ID:

219931

Transcript(s):

NM_139075.3 XM_005273824.3 XM_005273826.3 XM_005273830.3 XM_005273831.3 XM_005273832.3 XM_011544802.2 XM_011544807.2 XM_011544808.2 XM_017017328.1 XM_017017329.1 XM_017017330.1 XM_017017331.1 XM_017017332.1 XM_017017333.1 XM_017017334.1 XM_017017335.1 XM_017017336.1

Validation Results:

9%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00297554_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.