8899461
NM_003913.4 XM_011514970.2 XM_017011410.1 XM_017011411.1 XM_017011412.1 XM_017011413.1 XM_017011414.1 XM_017011415.1 XM_017011416.1 XM_017011417.1 XM_017011418.1 XM_017011419.1 XM_017011420.1 XM_017011421.1 XM_017011422.1 XM_017011423.1 XM_017011424.1 XM_017011425.1 XM_017011426.1 XM_017011427.1 XM_017011428.1 XM_017011429.1 XM_017011430.1 XM_017011431.1 XM_017011432.1 XM_017011433.1 XM_017011434.1 XM_017011435.1
20%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.