SiRNA Validation Data

siRNA ID:

5458

Gene ID:

10062

Transcript(s):

NM_001130102.2 NM_001251934.1 NM_001251935.1 NM_005693.3 XM_005252705.1 XM_005252706.1 XM_005252707.4 XM_005252709.1 XM_005252710.1 XM_005252715.2 XM_005252716.3 XM_006718112.1 XM_006718113.1 XM_006718115.1 XM_006718116.1 XM_011519805.2 XM_011519807.1 XM_011519808.2 XM_017017056.1 XM_017017057.1 XM_017017058.1

Validation Results:

5%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.