SiRNA Validation Data

siRNA ID:

s11429

Gene ID:

5723

Transcript(s):

NM_004577.3 XM_005271773.1 XM_005271774.1 XM_005271775.1 XM_005271776.1 XM_006715760.1 XM_006715761.1 XM_011515461.1 XM_017012466.1 XM_017012467.1 XM_017012468.1 XM_017012469.1

Validation Results:

17%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00190154_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.