SiRNA Validation Data

siRNA ID:

s11546

Gene ID:

5792

Transcript(s):

XM_005271079.3 XM_005271080.3 XM_005271081.3 XM_005271082.3 XM_006710795.3 XM_006710796.3 XM_006710797.3 XM_006710798.3 XM_006710799.3 XM_006710800.3 XM_006710801.3 XM_011541871.2 XM_011541872.2 XM_011541873.2 XM_017001942.1 XM_017001943.1 XM_017001944.1 XM_017001945.1 XM_017001946.1 XM_017001947.1 XM_017001948.1 XM_017001949.1 XM_017001950.1

Validation Results:

19%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00160858_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.