SiRNA Validation Data

siRNA ID:

s17018

Gene ID:

8899

Transcript(s):

NM_003913.4 XM_011514970.2 XM_017011410.1 XM_017011411.1 XM_017011412.1 XM_017011413.1 XM_017011414.1 XM_017011415.1 XM_017011416.1 XM_017011417.1 XM_017011418.1 XM_017011419.1 XM_017011420.1 XM_017011421.1 XM_017011422.1 XM_017011423.1 XM_017011424.1 XM_017011425.1 XM_017011426.1 XM_017011427.1 XM_017011428.1 XM_017011429.1 XM_017011430.1 XM_017011431.1 XM_017011432.1 XM_017011433.1 XM_017011434.1 XM_017011435.1

Validation Results:

18%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00907627_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.