SiRNA Validation Data

siRNA ID:

s19149

Gene ID:

9885

Transcript(s):

NM_001278649.1 NM_014835.3 NM_144498.2 XM_017028163.1 XM_017028164.1 XM_017028165.1 XM_017028166.1 XM_017028167.1 XM_017028168.1 XM_017028169.1 XM_017028170.1

Validation Results:

12%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00375886_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.