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SiRNA Validation Data

siRNA ID:

s194481

Gene ID:

2987

Transcript(s):

NM_000858.5 NM_001159390.1 NM_001159391.1 NM_001242839.1 XM_005273104.4 XM_017001092.1 XM_017001095.1 XM_017001096.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176133_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.