SiRNA Validation Data

siRNA ID:

s19568

Gene ID:

10062

Transcript(s):

NM_001130101.2 NM_001130102.2 NM_001251934.1 NM_001251935.1 NM_005693.3 XM_005252705.1 XM_005252706.1 XM_005252707.4 XM_005252709.1 XM_005252710.1 XM_005252713.3 XM_005252715.2 XM_005252716.3 XM_005252718.3 XM_006718112.1 XM_006718113.1 XM_006718115.1 XM_006718116.1 XM_011519805.2 XM_011519806.1 XM_011519807.1 XM_011519808.2 XM_017017058.1

Validation Results:

10%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00172885_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.