SiRNA Validation Data

siRNA ID:

s20136

Gene ID:

10298

Transcript(s):

NM_001014831.2 NM_001014832.1 NM_001014834.2 NM_001014835.1 NM_005884.3 XM_011526316.1 XM_011526317.2 XM_011526318.2 XM_011526319.2 XM_011526320.1

Validation Results:

11%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00178686_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.