SiRNA Validation Data

siRNA ID:

s2829

Gene ID:

1022

Transcript(s):

NM_001324069.1 NM_001324070.1 NM_001324071.1 NM_001324072.1 NM_001324074.1 NM_001324075.1 NM_001324077.1 NM_001324078.1 NM_001799.3 NR_136690.1 XM_011543094.2

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00387062_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.