SiRNA Validation Data

siRNA ID:

s29656

Gene ID:

54878

Transcript(s):

NM_001320875.1 NM_001320876.1 NM_017743.5 NM_130434.4 NM_197960.3 NM_197961.3 NR_135485.1 NR_135486.1 XM_005254505.1 XM_011521730.1 XM_011521731.1 XM_011521732.2 XM_011521733.1 XM_011521734.1 XM_017022373.1 XM_017022374.1 XM_017022375.1 XM_017022376.1 XM_017022377.1 XM_017022378.1 XM_017022379.1 XM_017022380.1 XM_017022381.1 XM_017022382.1 XM_017022383.1

Validation Results:

12%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00214745_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.