SiRNA Validation Data

siRNA ID:

s30648

Gene ID:

55288

Transcript(s):

NM_001033566.2 NM_001033567.2 NM_001033568.2 NM_001288754.1 NM_001288755.1 NM_001288758.1 NM_018307.4 NR_110083.1 XM_011524969.2 XM_011524971.2 XM_011524973.2 XM_011524975.2 XM_011524976.2 XM_017024819.1 XM_017024820.1 XM_017024821.1 XM_017024822.1 XM_017024823.1 XM_017024824.1 XM_017024825.1

Validation Results:

11%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00430266_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.