SiRNA Validation Data

siRNA ID:

s44445

Gene ID:

140885

Transcript(s):

NM_001040022.1 NM_001040023.1 NM_080792.2 XM_005260669.3 XM_005260670.3 XM_006723545.3 XM_011529173.2

Validation Results:

19%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00757426_s1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.