SiRNA Validation Data

siRNA ID:

s495

Gene ID:

9886

Transcript(s):

NM_001242359.1 NM_014836.4 NR_024554.2 NR_024555.1 XM_006718084.1 XM_006718085.1 XM_011540424.1 XM_011540425.1 XM_011540426.1 XM_011540428.1 XM_017017000.1 XM_017017001.1 XM_017017002.1 XM_017017003.1 XM_017017004.1 XM_017017005.1 XM_017017006.1 XM_017017007.1 XM_017017008.1

Validation Results:

11%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00323872_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.