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SiRNA Validation Data

siRNA ID:

104912

Gene ID:

8898

Transcript(s):

NM_001243571.1 NM_016156.5 NM_201278.2 NM_201281.2 XM_005274374.2 XM_005274375.2 XM_006718934.2 XM_006718935.2 XM_006718936.3 XM_011543058.2 XM_011543059.2 XM_017018517.1 XM_017018518.1

Validation Results:

9%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00269534_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.