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SiRNA Validation Data

siRNA ID:

1549

Gene ID:

6198

Transcript(s):

NM_001272042.1 NM_001272043.1 NM_001272044.1 NM_001272060.1 NM_003161.3 XM_011525101.2 XM_011525102.2 XM_011525103.2 XM_017024929.1 XM_017024930.1 XM_017024931.1 XM_017024932.1 XM_017024933.1

Validation Results:

23%(+/-0% mRNA remaining)(DU145 cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.