SiRNA Validation Data

siRNA ID:

445

Gene ID:

8569

Transcript(s):

NM_001135553.2 NM_003684.5 NM_198973.3 NR_024174.2 NR_024176.2 XM_006711000.1 XM_006711001.2 XM_006711002.2 XM_006711003.1 XM_006711013.1 XM_006711014.1 XM_006711015.1 XM_017002655.1 XM_017002656.1 XM_017002659.1 XM_017002660.1 XM_017002661.1 XM_017002662.1 XM_017002663.1 XM_017002664.1 XM_017002666.1 XM_017002667.1

Validation Results:

9%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.