SiRNA Validation Data



Gene ID:



NM_005037.5 NM_015869.4 NM_138711.3 NM_138712.3 XM_011533841.2 XM_011533842.2 XM_011533843.2 XM_011533844.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00234592_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.