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SiRNA Validation Data

siRNA ID:

s11751

Gene ID:

5894 5906

Transcript(s):

NM_002880.3 XM_005265355.2 XM_005265357.2 XM_005265358.4 XM_005265359.4 XM_011533974.2 XM_011533975.2 XM_017006966.1

Validation Results:

14%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00234119_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.