SiRNA Validation Data

siRNA ID:

s16278

Gene ID:

8550

Transcript(s):

NM_003668.3 NM_139078.2 XM_017020125.1 XM_017020126.1 XM_017020127.1 XM_017020128.1 XM_017020129.1 XM_017020130.1 XM_017020131.1 XM_017020132.1 XM_017020133.1 XM_017020134.1 XM_017020135.1 XM_017020136.1 XM_017020137.1 XM_017020138.1 XM_017020139.1

Validation Results:

18%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00177572_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.