SiRNA Validation Data



Gene ID:



NM_001300769.1 NM_001300770.1 NM_001300771.1 NM_001300772.1 NM_138794.4 XM_006711162.3 XM_011509182.2 XM_011509183.2 XM_017000271.1 XM_017000272.1

Validation Results:

14%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00371117_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.