SiRNA Validation Data

siRNA ID:

s673

Gene ID:

48

Transcript(s):

NM_001278352.1 NM_002197.2 XM_005251476.1 XM_011517888.1

Validation Results:

6%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00158095_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.