SiRNA Validation Data

siRNA ID:

s8514

Gene ID:

4140

Transcript(s):

NM_001128918.2 NM_001128919.2 NM_001128920.2 NM_001128921.2 NM_002376.6 XM_005267641.4 XM_005267642.4 XM_006720146.3 XM_011536769.2 XM_017021291.1 XM_017021292.1 XM_017021293.1 XM_017021294.1 XM_017021295.1 XM_017021296.1 XM_017021297.1 XM_017021298.1 XM_017021299.1 XM_017021300.1 XM_017021301.1 XM_017021302.1 XM_017021303.1 XM_017021304.1 XM_017021305.1 XM_017021306.1 XM_017021307.1 XM_017021308.1 XM_017021309.1 XM_017021310.1 XM_017021311.1

Validation Results:

7%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00159065_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.