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SiRNA Validation Data

siRNA ID:

s39922

Gene ID:

85363

Transcript(s):

NM_033034.2 NM_033092.2 NM_033093.2 XM_005253183.2 XM_005253184.2 XM_006718358.2 XM_011520426.2 XM_011520427.1 XM_017018460.1 XM_017018461.1 XM_017018462.1 XM_017018463.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs01552559_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.