SiRNA Validation Data

siRNA ID:

105180

Gene ID:

Transcript(s):

NM_015017.4 NM_201624.2 NM_201626.2 XM_005270648.1 XM_005270649.1 XM_011541055.1 XM_011541056.2 XM_017000722.1 XM_017000723.1 XM_017000724.1 XM_017000725.1 XM_017000726.1 XM_017000727.1 XM_017000728.1 XM_017000729.1

Validation Results:

7%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00367269_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.