SiRNA Validation Data

siRNA ID:

s37074

Gene ID:

80216

Transcript(s):

NM_001102406.1 NM_001253884.1 NM_025144.3 XM_005263245.4 XM_005263246.4 XM_006714326.3 XM_017008633.1 XM_017008634.1 XM_017008635.1 XM_017008636.1 XM_017008637.1 XM_017008638.1 XM_017008639.1 XM_017008640.1 XM_017008641.1 XM_017008642.1 XM_017008643.1 XM_017008644.1 XM_017008645.1 XM_017008646.1 XM_017008647.1 XM_017008648.1 XM_017008649.1 XM_017008650.1 XM_017008651.1

Validation Results:

19%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00228473_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.