Close X

SiRNA Validation Data

siRNA ID:

110610

Gene ID:

2821

Transcript(s):

NM_000175.3 NM_001184722.1 NM_001289789.1 NM_001289790.1 XM_005258764.2 XM_006723148.2 XM_011526754.2

Validation Results:

20%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00164752_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.