548991585
NM_001289095.1 NM_001289096.1 NM_001289098.1 NM_001289099.1 NM_001289100.1 NM_017771.4 XM_005265250.4 XM_005265252.4 XM_005265255.3 XM_005265256.3 XM_017006671.1 XM_017006672.1 XM_017006673.1 XM_017006674.1 XM_017006675.1 XM_017006676.1 XM_017006677.1 XM_017006678.1 XM_017006679.1 XM_017006680.1 XM_017006681.1 XM_017006682.1 XM_017006683.1 XM_017006684.1 XM_017006685.1 XM_017006686.1 XM_017006687.1 XM_017006688.1 XM_017006689.1 XM_017006690.1 XM_017006692.1 XM_017006693.1 XM_017006694.1 XM_017006695.1
13%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.