SiRNA Validation Data

siRNA ID:

s10615

Gene ID:

5329

Transcript(s):

NM_001005376.2 NM_001005377.2 NM_001301037.1 NM_002659.3 XM_005258990.4 XM_011527027.2 XM_011527028.2 XM_011527029.1 XM_011527030.2 XM_011527031.2 XM_017026872.1 XM_017026873.1

Validation Results:

17%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00182181_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.